Nucleic Acid Diagnostics

Current Research

Nucleic acid amplification tests are the 
gold standard for many infectious disease
 diagnoses due to high sensitivity and specificity, rapid operation, and low limits of detection. Despite the advantages of nucleic acid amplification tests, they currently offer limited point-of-care
 (POC) utility due to the need for complex instruments and laborious sample preparation. We report the development of the Nucleic Acid Isotachophoresis LAMP (NAIL) 
diagnostic device. NAIL uses isotachophoresis (ITP)
 and loop-mediated isothermal amplification (LAMP) to
 extract and amplify nucleic acids from complex matrices 
in less than one hour inside of an integrated chip. ITP is an
 electrokinetic separation technique that uses an electric
 field and two buffers to extract and purify nucleic acids in a single step. LAMP amplifies nucleic 
acids at constant temperature and produces large
 amounts of DNA that can be easily detected. The NAIL device potentially offers significant reductions in the complexity and cost of traditional nucleic acid diagnostics for POC applications.